Single-shot quantitative dispersion phase microscopy
نویسندگان
چکیده
منابع مشابه
Single-shot quantitative dispersion phase microscopy.
We present an imaging modality capable of providing high-speed optical dispersion measurements of live cells. The technique permits wide-field measurement of the optical phase shifts introduced by a sample for multiple discrete wavelengths in a single image capture. Utilizing spatial modulation and the wavelength dependence of the interference-fringe spacing, average refractive index as a funct...
متن کاملNoninterferometric single-shot quantitative phase microscopy.
We present a noninterferometric single-shot quantitative phase microscopy technique with the use of the transport of intensity equation (TIE). The optical configuration is based on a Michelson-like architecture attached to a nonmodified inverted transmission bright field microscope. Two laterally separated images from different focal planes can be obtained simultaneously by a single camera expo...
متن کاملSingle-shot Full-field reflection phase microscopy
We present a full-field reflection phase microscope that combines low-coherence interferometry and off-axis digital holographic microscopy (DHM). The reflection-based DHM provides highly sensitive and a single-shot imaging of cellular dynamics while the use of low coherence source provides a depth-selective measurement. The setup uniquely uses a diffraction grating in the reference arm to gener...
متن کاملSingle-shot quantitative phase microscopy with color-multiplexed differential phase contrast (cDPC)
We present a new technique for quantitative phase and amplitude microscopy from a single color image with coded illumination. Our system consists of a commercial brightfield microscope with one hardware modification-an inexpensive 3D printed condenser insert. The method, color-multiplexed Differential Phase Contrast (cDPC), is a single-shot variant of Differential Phase Contrast (DPC), which re...
متن کاملOptical detection and measurement of living cell morphometric features with single-shot quantitative phase microscopy.
We present a quadriwave lateral shearing interferometer used as a wavefront sensor and mounted on a commercial non-modified transmission white-light microscope as a quantitative phase imaging technique. The setup is designed to simultaneously make measurements with both quantitative transmission phase and fluorescence modes: phase enables enhanced contrasted visualization of the cell structure ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied Physics Letters
سال: 2012
ISSN: 0003-6951,1077-3118
DOI: 10.1063/1.4745785